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Journal article

Tracking low-copy transcription factors in living bacteria: the case of the lac repressor

Abstract:

Transcription factors control the expression of genes by binding to specific sites in DNA and repressing or activating transcription in response to stimuli. The lac repressor (LacI) is a well characterized transcription factor that regulates the ability of bacterial cells to uptake and metabolize lactose. Here, we study the intracellular mobility and spatial distribution of LacI in live bacteria using photoactivated localization microscopy combined with single-particle tracking. Since we trac...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1016/j.bpj.2017.02.028

Authors


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Role:
Author
ORCID:
0000-0001-5685-0352
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Institution:
University of Oxford
Division:
MPLS
Department:
Physics
Sub department:
Condensed Matter Physics
Oxford college:
St Cross College
Role:
Author
Consejo Nacional de Ciencia y Tecnología More from this funder
Engineering and Physical Sciences Research Council More from this funder
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Publisher:
Cell Press Publisher's website
Journal:
Biophysical Journal Journal website
Volume:
112
Issue:
7
Pages:
1316-1327
Publication date:
2017-04-11
Acceptance date:
2017-02-16
DOI:
EISSN:
1542-0086
ISSN:
0006-3495
Pmid:
28402875
Language:
English
Pubs id:
pubs:691145
UUID:
uuid:eb971c5b-d2ef-4f73-b5e5-d7488d839f31
Local pid:
pubs:691145
Deposit date:
2018-02-27

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