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The rate-limiting step of DNA synthesis by DNA polymerase occurs in the fingers-closed conformation

Abstract:

DNA polymerases maintain genomic integrity by copying DNA with high fidelity, part of which relies on the polymerase fingers opening-closing transition, a series of conformational changes during the DNA synthesis reaction cycle. Fingers opening and closing has been challenging to study, mainly due to the need to synchronise molecular ensembles. We previously studied fingers opening-closing on single polymerase-DNA complexes using single-molecule FRET; however, our work was limited to pre-chem...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1016/j.jmb.2021.167410

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Institution:
University of Oxford
Division:
MPLS
Department:
Physics
Sub department:
Condensed Matter Physics
Oxford college:
St Cross College
Role:
Author
ORCID:
0000-0001-6699-136X
Publisher:
Elsevier Publisher's website
Journal:
Journal of Molecular Biology Journal website
Volume:
434
Issue:
2
Article number:
167410
Publication date:
2021-12-17
Acceptance date:
2021-12-12
DOI:
ISSN:
0022-2836
Language:
English
Keywords:
Pubs id:
1226836
Local pid:
pubs:1226836
Deposit date:
2021-12-23

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