Journal article
Cross-linking mass spectrometry identifies new interfaces of Augmin required to localise the γ-Tubulin Ring Complex to the mitotic spindle
- Abstract:
-
The hetero-octameric protein complex, Augmin, recruits γ-Tubulin Ring Complex (γ-TuRC) to pre-existing microtubules (MTs) to generate branched MTs during mitosis, facilitating robust spindle assembly. However, despite a recent partial reconstitution of the human Augmin complex in vitro, the molecular basis of this recruitment remains unclear. Here, we used immuno-affinity purification of in vivo Augmin from Drosophila and cross-linking/mass spectrometry to identify distance restraints between...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
Actions
Authors
Funding
+ University of Exeter
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Funding agency for:
Metz, J
Wakefield, J
Grant:
CLES Strategic Development Fund Award
+ Wellcome Trust
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Funding agency for:
Chen, Z
Rappsiber, J
Grant:
108504
108504
+ Biotechnology and Biological Sciences Research Councils
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Funding agency for:
Chen, J
Grant:
BB/K017837/1
+ University of Exeter / Taiwan President's PhD studentship
More from this funder
Funding agency for:
Chen, J
Grant:
BB/K017837/1
Bibliographic Details
- Publisher:
- Company of Biologists Publisher's website
- Journal:
- Biology Open Journal website
- Volume:
- 2017
- Issue:
- 6
- Pages:
- 654-663
- Publication date:
- 2017-03-28
- Acceptance date:
- 2017-03-16
- DOI:
- ISSN:
-
2046-6390
Item Description
- Pubs id:
-
pubs:686667
- UUID:
-
uuid:d113b251-554b-407e-918b-9fa9bd71d2d2
- Local pid:
- pubs:686667
- Source identifiers:
-
686667
- Deposit date:
- 2017-03-22
Terms of use
- Copyright holder:
- © 2017 Published by The Company of Biologists Ltd
- Copyright date:
- 2017
- Notes:
-
© 2017. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
- Licence:
- CC Attribution (CC BY)
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