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Monitoring real-time hormone release kinetics via high-content 3-D imaging of compensatory endocytosis

Abstract:

High-content real-time imaging of hormone secretion in tissues or cell populations is a challenging task, which is unlikely to be resolved directly, despite immense translational value. We approach this problem indirectly, using compensatory endocytosis, a process that closely follows exocytosis in the cell, as a surrogate read-out for secretion. The tissue is immobilized in an open-air perifusion chamber and imaged using a two-photon microscope. A fluorescent polar tracer, perifused through ...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1039/c8lc00417j

Authors


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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM
Sub department:
OCDEM
Oxford college:
St Catherine's College
Role:
Author
ORCID:
0000-0002-8883-176X
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM
Sub department:
OCDEM
Role:
Author
ORCID:
0000-0002-8263-2460
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM
Sub department:
OCDEM
Role:
Author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM
Sub department:
OCDEM
Role:
Author
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Funding agency for:
Rorsman, P
Grant:
Senior Investigator Award 095531
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Funding agency for:
Hamilton, A
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Funding agency for:
Tarasov, A
Publisher:
Royal Society of Chemistry Publisher's website
Journal:
Lab on a Chip Journal website
Volume:
18
Issue:
18
Pages:
2838-2848
Publication date:
2018-07-30
Acceptance date:
2018-07-26
DOI:
EISSN:
1473-0189
ISSN:
1473-0197
Pmid:
30083680
Source identifiers:
896889
Language:
English
Pubs id:
pubs:896889
UUID:
uuid:b42faecd-a9ca-4d33-9445-c80dc224b565
Local pid:
pubs:896889
Deposit date:
2018-09-05

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