Thesis
Engineered α-hemolysin pores with chemically and genetically-fused functional proteins
- Abstract:
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Protein engineering could be used to bring two proteins together, which don't normally interact, in an oriented configuration. Using computer modelling and experimental work involving mutagenesis, a new dimer complex, (α7)2, was engineered with two α-hemolysin (αHL) heptamers (α7) units linked via disulfide bridges in a cap-to-cap orientation. The structure of (α7)2 was confirmed by biochemical analysis, transmission electron microscopy (TEM) and single-channel electrical recording. Import...
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Bibliographic Details
- Publication date:
- 2013
- Type of award:
- DPhil
- Level of award:
- Doctoral
- Awarding institution:
- Oxford University, UK
Item Description
- Language:
- English
- Subjects:
- UUID:
-
uuid:ac99948c-9c3a-4388-a2fc-5955fd9446ee
- Local pid:
- ora:7145
- Deposit date:
- 2013-08-07
Terms of use
- Copyright holder:
- Mantri, S; Shiksha Mantri
- Copyright date:
- 2013
- Notes:
- This thesis is not currently available in ORA.
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