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Journal article

Structural basis of transcription inhibition by fidaxomicin (lipiarmycin A3)

Abstract:

Fidaxomicin is an antibacterial drug in clinical use for treatment of Clostridium difficile diarrhea. The active ingredient of fidaxomicin, lipiarmycin A3 (Lpm), functions by inhibiting bacterial RNA polymerase (RNAP). Here we report a cryo-EM structure of Mycobacterium tuberculosis RNAP holoenzyme in complex with Lpm at 3.5-Å resolution. The structure shows that Lpm binds at the base of the RNAP "clamp." The structure exhibits an open conformation of the RNAP clamp, suggesting that Lpm traps...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1016/j.molcel.2018.02.026

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Institution:
University of Oxford
Division:
MPLS Division
Department:
Physics; Condensed Matter Physics
Role:
Author
Publisher:
Cell Press Publisher's website
Journal:
Molecular Cell Journal website
Volume:
70
Issue:
1
Pages:
60-71.e15
Publication date:
2018-03-28
Acceptance date:
2018-02-23
DOI:
EISSN:
1097-4164
ISSN:
1097-2765
Pmid:
29606590
Language:
English
Keywords:
Pubs id:
pubs:833421
UUID:
uuid:6fed87e6-852f-4fa0-adaf-17531a31ebb8
Local pid:
pubs:833421
Source identifiers:
833421
Deposit date:
2018-04-05

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