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Journal article

Single molecule fluorescence in situ hybridisation for quantitating post-transcriptional regulation in Drosophila brains.

Abstract:

RNA in situ hybridization is a powerful method to investigate post-transcriptional regulation, but analysis of intracellular mRNA distributions in thick, complex tissues like the brain poses significant challenges. Here, we describe the application of single-molecule fluorescent in situ hybridization (smFISH) to quantitate primary nascent transcription and post-transcriptional regulation in whole-mount Drosophila larval and adult brains. Combining immunofluorescence and smFISH probes for diff...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1016/j.ymeth.2017.06.025

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Role:
Author
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Fondation Bettencourt Schueller More from this funder
More from this funder
Grant:
091911/B/10/Z
107457/Z/15/Z
081858
MICRON Oxford More from this funder
Clarendon Trust More from this funder
Goodger Fund More from this funder
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Publisher:
Elsevier Publisher's website
Journal:
Methods Journal website
Volume:
126
Pages:
166-176
Publication date:
2017-06-24
Acceptance date:
2017-06-21
DOI:
EISSN:
1095-9130
ISSN:
1046-2023
Language:
English
Keywords:
Pubs id:
pubs:702416
UUID:
uuid:6bb02d29-d0ce-4217-8a68-660da79c1dde
Local pid:
pubs:702416
Deposit date:
2017-08-11

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