Journal article
Single-molecule and super-resolution imaging of transcription in living bacteria
- Abstract:
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In vivo single-molecule and super-resolution techniques are transforming our ability to study transcription as it takes place in its native environment in living cells. This review will detail the methods for imaging single molecules in cells, and the data-analysis tools which can be used to extract quantitative information on the spatial organization, mobility, and kinetics of the transcription machinery from these experiments. Furthermore, we will highlight studies which have applied these ...
Expand abstract
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Version of record, pdf, 2.1MB)
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- Publisher copy:
- 10.1016/j.ymeth.2017.04.001
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Authors
Funding
+ Biotechnology and Biological Sciences Research Council
More from this funder
Grant:
BB/J00054X/1
BB/N018656/1
+ Engineering and Physical Sciences Research Council
More from this funder
Grant:
University of Oxford LSI-DTC
Bibliographic Details
- Publisher:
- Elsevier Publisher's website
- Journal:
- Methods Journal website
- Volume:
- 120
- Pages:
- 103-114
- Publication date:
- 2017-04-13
- Acceptance date:
- 2017-04-05
- DOI:
- EISSN:
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1095-9130
- ISSN:
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1046-2023
- Pmid:
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28414097
Item Description
- Language:
- English
- Keywords:
- Pubs id:
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pubs:691144
- UUID:
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uuid:68f81e77-eb6c-43b9-b2e0-af408c1a9582
- Local pid:
- pubs:691144
- Deposit date:
- 2018-02-27
Terms of use
- Copyright holder:
- M Stracy and AN Kapanidis
- Copyright date:
- 2017
- Notes:
- © 2017 The Authors. Published by Elsevier Inc.
- Licence:
- CC Attribution (CC BY)
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