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How to tackle challenging ChIP-Seq, with long-range cross-linking, using ATRX as an example

Abstract:

Chromatin immunoprecipitation coupled with high-throughput, next-generation DNA sequencing (ChIP-seq) has enabled researchers to establish the genome-wide patterns of chromatin modifications and binding of chromatin-associated proteins. Well-established protocols produce robust ChIP-seq data for many proteins by sequencing the DNA obtained following immunoprecipitation of fragmented chromatin using a wide range of specific antibodies. In general, the quality of these data mainly depends on th...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1007/978-1-4939-8663-7_6

Authors


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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM
Sub department:
Weatherall Institute of Molecular Medicine
Oxford college:
St Edmund Hall
Role:
Author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM
Sub department:
Weatherall Institute of Molecular Medicine
Role:
Author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM
Sub department:
Weatherall Institute of Molecular Medicine
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
RDM
Sub department:
RDM Strategic
Oxford college:
Green Templeton College
Role:
Author
ORCID:
0000-0002-7680-9311
Publisher:
Springer Publisher's website
Pages:
105-130
Series:
Methods in Molecular Biology
Host title:
Methods in Molecular Biology
Publication date:
2018-08-03
Acceptance date:
2018-06-05
DOI:
EISSN:
1940-6029
ISSN:
1064-3745
Pmid:
30073524
Source identifiers:
905109
Language:
English
Keywords:
Pubs id:
pubs:905109
UUID:
uuid:64a219a8-1fcd-4f55-ad54-cc862764f2aa
Local pid:
pubs:905109
Deposit date:
2018-12-04

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