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Journal article

Isolation of Leishmania promastigote flagella

Abstract:

Eukaryotic flagella are conserved multifunctional organelles with roles in motility, intercellular interactions and signal transduction. Leishmania possess a single flagellum at all stages of their life cycle. Flagella of promastigote forms in the fly are long and motile, with a canonical 9+2 microtubule axoneme and an extra-axonemal paraflagellar rod (PFR). This protocol describes a simple method for the isolation of Leishmania mexicana promastigote flagella, optimised to yield intact flagel...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1007/978-1-0716-0294-2_29

Authors


More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Pathology Dunn School
Oxford college:
St Edmund Hall
Role:
Author
ORCID:
0000-0001-9117-2649
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Pathology Dunn School
Role:
Author
ORCID:
0000-0002-4270-8360
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Pathology Dunn School
Oxford college:
St Edmund Hall
Role:
Author
ORCID:
0000-0003-4346-8896
Publisher:
Humana Press Publisher's website
Pages:
485-495
Series:
Methods in Molecular Biology
Series number:
2116
Publication date:
2020-03-28
Acceptance date:
2019-04-25
DOI:
EISBN:
9781071602942
EISSN:
1940-6029
ISSN:
1064-3745
Source identifiers:
994615
ISBN:
9781071602935
Language:
English
Keywords:
Pubs id:
pubs:994615
UUID:
uuid:64161c05-567a-4797-a5bd-75f8523fc7c4
Local pid:
pubs:994615
Deposit date:
2019-05-15

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