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Blocking variant surface glycoprotein synthesis alters endoplasmic reticulum exit sites/Golgi homeostasis in Trypanosoma brucei

Abstract:

The predominant secretory cargo of bloodstream form Trypanosoma brucei is variant surface glycoprotein (VSG), comprising ~10% total protein and forming a dense protective layer. Blocking VSG translation using Morpholino oligonucleotides triggered a precise pre-cytokinesis arrest. We investigated the effect of blocking VSG synthesis on the secretory pathway. The number of Golgi decreased, particularly in post-mitotic cells, from 3.5 ± 0.6 to 2.0 ± 0.04 per cell. Similarly, the number of endopl...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1111/tra.12561

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Institution:
University of Oxford
Division:
MSD
Department:
Pathology Dunn School
Oxford college:
St Edmund Hall
Role:
Author
ORCID:
0000-0003-4346-8896
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Publisher:
Wiley Publisher's website
Journal:
Traffic Journal website
Volume:
19
Issue:
6
Pages:
391-405
Publication date:
2018-04-06
Acceptance date:
2018-03-08
DOI:
EISSN:
1600-0854
ISSN:
1398-9219
Pmid:
29533496
Source identifiers:
828989
Language:
English
Keywords:
Pubs id:
pubs:828989
UUID:
uuid:62d8ad59-827a-41ed-881b-a0615e66f770
Local pid:
pubs:828989
Deposit date:
2018-05-11

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