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Ultrasound-mediated DNA transfer for bacteria

Abstract:

In environmental microbiology, the most commonly used methods of bacterial DNA transfer are conjugation and electroporation. However, conjugation requires physical contact and cell-pilus-cell interactions; electroporation requires low-ionic strength medium and high voltage. These limitations have hampered broad applications of bacterial DNA delivery. We have employed a standard low frequency 40 kHz ultrasound bath to successfully transfer plasmid pBBR1MCS2 into Pseudomonas putida UWC1, Escher...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1093/nar/gkm710

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Institution:
"Centre for Ecology & Hydrology, Oxford", "Tsinghua University, Beijing, People's Republic of China"
Department:
Department of Environmental Science and Engineering
Role:
Author
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Institution:
"Centre for Ecology & Hydrology, Oxford"
Role:
Author
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Institution:
"Centre for Ecology & Hydrology, Oxford", "University of Oxford"
Department:
Begbroke Directorate
Role:
Author
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Institution:
"Coventry University"
Department:
Faculty of Health and Life Sciences
Role:
Author
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Institution:
University of Oxford
Research group:
Preston Group
Department:
Mathematical,Physical & Life Sciences Division - Plant Sciences
Role:
Author
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National Natural Science Foundation of China More from this funder
Publisher:
Oxford University Press Publisher's website
Journal:
Nucleic Acids Research Journal website
Volume:
35
Issue:
19
Pages:
Article: e129
Publication date:
2007-10-05
DOI:
EISSN:
1362-4962
ISSN:
0305-1048
URN:
uuid:5bb5f0b6-3d90-49d3-b5d1-1c3680261fda
Local pid:
ora:2825

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