Journal article
A CRISPR Cas9 high-throughput genome editing toolkit for kinetoplastids
- Abstract:
-
Clustered regularly interspaced short palindromic repeats (CRISPR), CRISPR-associated gene 9 (Cas9) genome editing is set to revolutionize genetic manipulation of pathogens, including kinetoplastids. CRISPR technology provides the opportunity to develop scalable methods for high-throughput production of mutant phenotypes. Here, we report development of a CRISPR-Cas9 toolkit that allows rapid tagging and gene knockout in diverse kinetoplastid species without requiring the user to perform any D...
Expand abstract
- Publication status:
- Published
- Peer review status:
- Peer reviewed
Actions
Access Document
- Files:
-
-
(Version of record, pdf, 1.5MB)
-
- Publisher copy:
- 10.1098/rsos.170095
Why is the content I wish to access not available via ORA?
Bibliographic data (the information relating to research outputs) and full-text items (e.g. articles, theses, reports, etc.) arrive in ORA from several different sources. Unfortunately we are not able to make available the full-text for every research output.
Please contact the ORA team (
) if you have queries regarding unavailable content OR if you are aware of a full-text copy we can make available.
) if you have queries regarding unavailable content OR if you are aware of a full-text copy we can make available.
Content may be unavailable for the following four reasons
-
Version unsuitable
-
We have not obtained a suitable full-text for a given research output. See this page for more information.
-
Recently completed
-
Sometimes content is held in ORA but is unavailable for a fixed period of time to comply with the policies and wishes of rights holders.
-
Permissions
-
All content made available in ORA should comply with relevant rights, such as copyright. See this page for more information.
-
Clearance
-
Some thesis volumes scanned as part of the digitisation scheme funded by Dr Leonard Polonsky are currently unavailable due to sensitive material or uncleared third-party copyright content. We are attempting to contact authors whose theses are affected.
Alternative access to the full-text
You may be able to access the full-text directly from the publisher's website using the 'Publisher Copy' link in the 'Links & Downloads' box from a research output's ORA record page. This method may require an institutional or individual subscription to the journal/resource.
Authors
Funding
+ Medical Research Council
More from this funder
Funding agency for:
Beneke, T
Grant:
15/16_MSD_836338
+ Medical Research Council
More from this funder
Funding agency for:
Valli, J
Grant:
13/14_MSD_OSS_363238
Expand funders...
Bibliographic Details
- Publisher:
- Royal Society Publisher's website
- Journal:
- Royal Society Open Science Journal website
- Volume:
- 4
- Issue:
- 5
- Pages:
- 170095
- Publication date:
- 2017-05-03
- Acceptance date:
- 2017-03-29
- DOI:
- ISSN:
-
2054-5703
- Pmid:
-
28573017
- Source identifiers:
-
688016
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:688016
- UUID:
-
uuid:5250e93d-9487-4df9-8610-bc33b1c55a2b
- Local pid:
- pubs:688016
- Deposit date:
- 2017-10-11
Terms of use
- Copyright holder:
- © 2017 Beneke, et al
- Copyright date:
- 2017
- Notes:
- Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
- Licence:
- CC Attribution (CC BY)
If you are the owner of this record, you can report an update to it here: Report update to this record