Journal article
Real-time intrinsic fluorescence visualization and sizing of proteins and protein complexes in microfluidic devices
- Abstract:
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Optical detection has become a convenient and scalable approach to read out information from microfluidic systems. For the study of many key biomolecules, however, including peptides and proteins, which have low fluorescence emission efficiencies at visible wavelengths, this approach typically requires labeling of the species of interest with extrinsic fluorophores to enhance the optical signal obtained - a process which can be time-consuming, requires purification steps, and has the propensi...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
-
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(Accepted manuscript, pdf, 6.2MB)
-
- Publisher copy:
- 10.1021/acs.analchem.7b04523
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Authors
Funding
Engineering and Physical Sciences Research Council
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Bibliographic Details
- Publisher:
- American Chemical Society Publisher's website
- Journal:
- Analytical Chemistry Journal website
- Volume:
- 90
- Issue:
- 6
- Pages:
- 3849-3855
- Publication date:
- 2018-02-16
- Acceptance date:
- 2018-02-16
- DOI:
- EISSN:
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1520-6882
- ISSN:
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0003-2700
- Pmid:
-
29451779
- Source identifiers:
-
827706
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:827706
- UUID:
-
uuid:52192bd1-97d2-45cb-a46f-a2ce3e23326c
- Local pid:
- pubs:827706
- Deposit date:
- 2018-03-03
Terms of use
- Copyright holder:
- American Chemical Society
- Copyright date:
- 2018
- Notes:
- © 2018 American Chemical Society. This is the accepted manuscript version of the article. The final version is available online from American Chemical Society at: 10.1021/acs.analchem.7b04523
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