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Journal article

Real-time intrinsic fluorescence visualization and sizing of proteins and protein complexes in microfluidic devices

Abstract:

Optical detection has become a convenient and scalable approach to read out information from microfluidic systems. For the study of many key biomolecules, however, including peptides and proteins, which have low fluorescence emission efficiencies at visible wavelengths, this approach typically requires labeling of the species of interest with extrinsic fluorophores to enhance the optical signal obtained - a process which can be time-consuming, requires purification steps, and has the propensi...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1021/acs.analchem.7b04523

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Role:
Author
ORCID:
0000-0002-0863-381X
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Role:
Author
ORCID:
0000-0002-8018-3059
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Engineering and Physical Sciences Research Council More from this funder
Publisher:
American Chemical Society Publisher's website
Journal:
Analytical Chemistry Journal website
Volume:
90
Issue:
6
Pages:
3849-3855
Publication date:
2018-02-16
Acceptance date:
2018-02-16
DOI:
EISSN:
1520-6882
ISSN:
0003-2700
Pmid:
29451779
Source identifiers:
827706
Language:
English
Keywords:
Pubs id:
pubs:827706
UUID:
uuid:52192bd1-97d2-45cb-a46f-a2ce3e23326c
Local pid:
pubs:827706
Deposit date:
2018-03-03

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