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Conference item

Single-cell measurements of two-dimensional binding affinity across cell contacts

Abstract:

The two-dimensional (2D) affinity between protein molecules across contacting cells is a key parameter regulating and initiating several cellular processes. However, measuring 2D affinity can be challenging and experimental data are limited. In addition, the obtained 2D affinities are typically averaged over the cell population. We here present a method to measure 2D affinity on single cells binding to polyhistidine-tagged fluorescent ligands anchored to a supported lipid bilayer (SLB). By de...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1016/j.bpj.2021.10.010

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Institution:
University of Oxford
Division:
MSD
Department:
RDM
Sub department:
RDM - Investigative Medicine Division
Role:
Author
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Publisher:
Cell Press Publisher's website
Journal:
Biophysical Journal Journal website
Volume:
120
Issue:
22
Pages:
5032-5040
Host title:
Biophysical journal
Publication date:
2021-10-12
Acceptance date:
2021-10-08
Event location:
United States
DOI:
EISSN:
1542-0086
ISSN:
0006-3495
Pmid:
34653390
Language:
English
Keywords:
Pubs id:
1192155
Local pid:
pubs:1192155
Deposit date:
2021-10-26

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