Journal article icon

Journal article

Effect of iASPP RNAi vector transfection on apoptosis of MCF-7

Abstract:

Objective: To observe the effect of the iASPP RNAi vector transfection on the apoptosis of breat cancer cell MCF-7. Methods: The specific siRNA sequence was designed according to the genebank. The sequence was cloned into PGCsilencer™ H1/Neo/GFP. The recombinant plasmid was transfected into MCF-7 by Lipofectamine™ 2000. The iASPP expression was analyzed by RT-PCR and Western blot. The cell apoptosis was detected by FCM. Results: The iASPP expression in MCF-7 cell lines was descended from 40% ...

Expand abstract

Actions


Authors


Expand authors...
Journal:
Chinese Journal of Cancer Prevention and Treatment
Volume:
15
Issue:
21
Publication date:
2008-11-01
ISSN:
1673-5269
Source identifiers:
84113
Language:
Chinese
Keywords:
Pubs id:
pubs:84113
UUID:
uuid:4cc987fc-798a-4f5d-9e81-42a849a62b57
Local pid:
pubs:84113
Deposit date:
2012-12-19

Terms of use


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP