Book section
Imaging of single dye-labeled chemotaxis proteins in live bacteria using electroporation
- Abstract:
-
For the last 2 decades, the use of genetically fused fluorescent proteins (FPs) has greatly contributed to the study of chemotactic signaling in E. coli, including the activation of the response regulator protein CheY and its interaction with the flagellar motor. However, this approach suffers from a number of limitations, both biological and biophysical. For example, not all fusions are fully functional when fused to a bulky FP, which can have a similar molecular weight to its fused counterp...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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Authors
Funding
+ Engineering and Physical Sciences Research Council
More from this funder
Funding agency for:
Di Paolo, D
Grant:
DTC studentship
Bibliographic Details
- Publisher:
- Humana Press Publisher's website
- Host title:
- Bacterial Chemosensing
- Series:
- Methods in Molecular Biology
- Volume:
- 1729
- Pages:
- 233-246
- Publication date:
- 2018-02-11
- Acceptance date:
- 2016-07-02
- DOI:
- ISSN:
-
1064-3745
- Pmid:
-
29429095
- ISBN:
- 9781493975761
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:826223
- UUID:
-
uuid:3be59432-20c8-4524-833a-c2db97893c70
- Local pid:
- pubs:826223
- Source identifiers:
-
826223
- Deposit date:
- 2018-04-04
Terms of use
- Copyright holder:
- Springer Science+Business Media, LLC
- Copyright date:
- 2018
- Notes:
- Copyright © 2018 Springer Science+Business Media, LLC.
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