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RASER-FISH: non-denaturing fluorescence in situ hybridization for preservation of three-dimensional interphase chromatin structure

Abstract:

DNA fluorescence in situ hybridization (FISH) has been a central technique in advancing our understanding of how chromatin is organized within the nucleus. With the increasing resolution offered by super-resolution microscopy, the optimal maintenance of chromatin structure within the nucleus is essential for accuracy in measurements and interpretation of data. However, standard 3D-FISH requires potentially destructive heat denaturation in the presence of chaotropic agents such as formamide to...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/s41596-022-00685-8

Authors


More by this author
Institution:
University of Oxford
Division:
MSD
Department:
RDM
Sub department:
RDM Clinical Laboratory Sciences
Oxford college:
Wolfson College
Role:
Author
ORCID:
0000-0003-1195-2608
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Grant:
MC_UU_00016/1
MR/N00969X/1
Publisher:
Springer Nature Publisher's website
Journal:
Nature Protocols Journal website
Publication date:
2022-04-04
Acceptance date:
2022-01-10
DOI:
EISSN:
1750-2799
ISSN:
1754-2189
Language:
English
Keywords:
Pubs id:
1234364
Local pid:
pubs:1234364
Deposit date:
2022-01-27

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