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Viral detection and identification in 20 min by rapid single-particle fluorescence in-situ hybridization of viral RNA

Abstract:

The increasing risk from viral outbreaks such as the ongoing COVID-19 pandemic exacerbates the need for rapid, affordable and sensitive methods for virus detection, identification and quantification; however, existing methods for detecting virus particles in biological samples usually depend on multistep protocols that take considerable time to yield a result. Here, we introduce a rapid fluorescence in situ hybridization (FISH) protocol capable of detecting influenza virus, avian infectious b...

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Publication status:
Published
Peer review status:
Peer reviewed

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Files:
Publisher copy:
10.1038/s41598-021-98972-z

Authors


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Institution:
University of Oxford
Division:
MPLS
Department:
Physics
Sub department:
Condensed Matter Physics
Role:
Author
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Institution:
University of Oxford
Division:
MSD
Sub department:
BR MEDICAL SCIENCES DIV OFFICE; HB NDM EXPERIMENTAL MEDICINE
Oxford college:
Harris Manchester College
Role:
Author
ORCID:
0000-0002-4036-4269
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University of Oxford COVID-19 Research Response Fund More from this funder
Publisher:
Springer Nature Publisher's website
Journal:
Scientific Reports Journal website
Volume:
11
Issue:
1
Article number:
19579
Publication date:
2021-10-01
Acceptance date:
2021-09-09
DOI:
EISSN:
2045-2322
Pmid:
34599242
Language:
English
Keywords:
Pubs id:
1198569
Local pid:
pubs:1198569
Deposit date:
2021-11-23

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