RNA polymerase pausing during initial transcription
In bacteria, RNA polymerase (RNAP) initiates transcription by synthesizing short transcripts that are either released or extended to allow RNAP to escape from the promoter. The mechanism of initial transcription is unclear due to the presence of transient intermediates and molecular heterogeneity. Here, we studied initial transcription on a lac promoter using single-molecule fluorescence observations of DNA scrunching on immobilized transcription complexes. Our work revealed a long pause ("in...Expand abstract
- Publication status:
- Peer review status:
- Peer reviewed
(Version of record, pdf, 4.0MB)
- Publisher copy:
- Copyright holder:
- Duchi et al
- Copyright date:
Copyright © 2016 The Authors. Published by Elsevier Inc.
This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
- CC Attribution (CC BY)
If you are the owner of this record, you can report an update to it here: Report update to this record