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RNA polymerase pausing during initial transcription

Abstract:

In bacteria, RNA polymerase (RNAP) initiates transcription by synthesizing short transcripts that are either released or extended to allow RNAP to escape from the promoter. The mechanism of initial transcription is unclear due to the presence of transient intermediates and molecular heterogeneity. Here, we studied initial transcription on a lac promoter using single-molecule fluorescence observations of DNA scrunching on immobilized transcription complexes. Our work revealed a long pause ("in...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1016/j.molcel.2016.08.011

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Institution:
University of Oxford
Division:
MPLS
Department:
Physics
Role:
Author
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Institution:
University of Oxford
Division:
MPLS
Department:
Physics
Sub department:
Condensed Matter Physics
Role:
Author
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Funding agency for:
Bauer, D
Grant:
EPAC Junior Research Fellowship
More from this funder
Funding agency for:
Bauer, D
Grant:
EPAC Junior Research Fellowship
Publisher:
Cell Press Publisher's website
Journal:
Molecular cell Journal website
Volume:
63
Issue:
6
Pages:
939-950
Publication date:
2016-09-03
DOI:
EISSN:
1097-4164
ISSN:
1097-2765
Pmid:
27618490
Language:
English
Keywords:
Pubs id:
pubs:645858
UUID:
uuid:288ee21f-f5ce-4fe4-b19b-2f1e5b45c6ef
Local pid:
pubs:645858
Deposit date:
2017-05-20

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