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Characterisation of Casein Kinase 1.1 in Leishmania donovani Using the CRISPR Cas9 Toolkit

Abstract:

The recent adaptation of CRISPR Cas9 genome editing to Leishmania spp. has opened a new era in deciphering Leishmania biology. The method was recently improved using a PCR-based CRISPR Cas9 approach, which eliminated the need for cloning. This new approach, which allows high-throughput gene deletion, was successfully validated in L. mexicana and L. major. In this study, we validated the toolkit in Leishmania donovani targeting the flagellar protein PF16, confirming that the tagged protein loc...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1155/2017/4635605

Authors


More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Pathology Dunn School
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Pathology Dunn School
Oxford college:
St Edmund Hall
Role:
Author
ORCID:
0000-0003-4346-8896
Publisher:
Hindawi Publishing Corporation Publisher's website
Journal:
BioMed Research International Journal website
Volume:
2017
Pages:
1-11
Publication date:
2017-11-29
Acceptance date:
2017-10-12
DOI:
EISSN:
2314-6141
ISSN:
2314-6133
Source identifiers:
810297
Keywords:
Pubs id:
pubs:810297
UUID:
uuid:1c24a95c-3534-4834-a2c8-cc25393feff5
Local pid:
pubs:810297
Deposit date:
2017-12-10

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