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Thesis

Investigating cell type specific metabolism using GFP as a reporter protein

Abstract:

Metabolic flux analysis (MFA) is a powerful technique for quantifying the intracellular fluxes in central carbon metabolism. It relies on detection of stable isotope labelling from metabolites such as amino acids derived from protein. Current standard techniques are, however, unable to distinguish between different cell types in heterogeneous tissue. The aim of the thesis was to address this problem by developing and validating a strategy using green fluorescent protein (GFP) with cell typ...

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Institution:
University of Oxford
Oxford college:
Wolfson College
Role:
Author
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Division:
MPLS
Department:
Plant Sciences
Role:
Author, Supervisor
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Division:
MPLS
Department:
Plant Sciences
Role:
Author, Supervisor
Biotechnology and Biological Sciences Research Council More from this funder
Type of award:
DPhil
Level of award:
Doctoral
Awarding institution:
University of Oxford
UUID:
uuid:0c418362-63e7-496d-9ff6-584a0c54c127
Deposit date:
2015-11-11

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