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Journal article

Mechanism of nucleic acid unwinding by SARS-CoV helicase

Abstract:

The non-structural protein 13 (nsp13) of Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) is a helicase that separates double-stranded RNA (dsRNA) or DNA (dsDNA) with a 5′→3′ polarity, using the energy of nucleotide hydrolysis. We determined the minimal mechanism of helicase function by nsp13. We showed a clear unwinding lag with increasing length of the double-stranded region of the nucleic acid, suggesting the presence of intermediates in the unwinding process. To elucidate the natu...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1371/journal.pone.0036521

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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Pathology Dunn School
Role:
Author
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Publisher:
Public Library of Science Publisher's website
Journal:
PloS ONE Journal website
Volume:
7
Issue:
5
Pages:
ARTN e36521
Publication date:
2012-05-15
Acceptance date:
2012-04-03
DOI:
EISSN:
1932-6203
Source identifiers:
375229
Language:
English
Keywords:
UUID:
uuid:04ba5246-8f2a-4eb3-96ae-b4f1377ef3b0
Local pid:
pubs:375229
Deposit date:
2013-11-16

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