A semi-automated method for counting fluorescent malaria oocysts increases the throughput of transmission blocking studies.
|Abstract||Malaria transmission is now recognized as a key target for intervention. Evaluation of the Plasmodium oocyst burden in the midguts of Anopheles spp. is important for many of assays investigating transmission. However, current assays are very time-consuming, manually demanding and patently subject to observer-observer variation.|
|Author||Delves, MJ; Sinden, RE;|
|Key phrase||Animals Anopheles Antimalarials Automation Dehydroepiandrosterone Sulfate Ethanolamines Female Fluorenes Green Fluorescent Proteins Humans Image Processing, Computer-Assisted Malaria Male Mice Microscopy Oocysts Plasmodium berghei Reproducibility of Results Sensitivity and Specificity|
Computational analysis of binding between malarial dihydrofolate reductases and anti-folates.
|Abstract||Plasmodium falciparum readily develops resistance to the anti-folates pyrimethamine and proguanil via a characteristic set of mutations in the dihydrofolate reductase (PfDHFR) gene that leads to reduced competitive drug binding at the enzyme's active site. Analogous mutations can be found in the DHFR gene in isolates of Plasmodium vivax (PvDHFR) although anti-folates have not been widely used for the treatment of this infection. Here the interact ... [truncated at 450 characters in length]|
|Author||Choowongkomon, K; Theppabutr, S; Songtawee, N; et al|
|Key phrase||Amino Acid Sequence Animals Drug Resistance Folic Acid Antagonists Models, Molecular Molecular Sequence Data Mutation Plasmodium malariae Plasmodium ovale Pyrimethamine Sequence Analysis Structural Homology, Protein Tetrahydrofolate Dehydrogenase|
Specific role for cathepsin S in the generation of antigenic peptides in vivo.
|Abstract||To address the role of different proteases in degradation of antigen destined for MHC class II-restricted presentation, we generated cathepsin-deficient mice carrying a transgenic B cell receptor (BCR) specific for hen egg lysozyme (HEL). We demonstrate that degradation of HEL in B lymphocytes is highly processive and does not result in discrete processing intermediates. Moreover, degradation of HEL does not require initial unlocking of the antig ... [truncated at 450 characters in length]|
|Author||Plüger, EB; Boes, M; Alfonso, C; et al|
|Key phrase||Animals Antigen Presentation Cathepsin B Cathepsin L Cathepsins Chickens Cysteine Endopeptidases Epitopes Mice Mice, Inbred C57BL Mice, Knockout Mice, Transgenic Muramidase Peptide Fragments Protein Biosynthesis Protein Processing, Post-Translational Receptors, Antigen, B-Cell|
Expression of myelin proteins in the opossum optic nerve: late appearance of inhibitors implicates an earlier non-myelin factor in preventing ganglion cell regeneration.
|Abstract||The pattern of appearance of myelin-associated proteins in the visual system of the Brazilian opossum Monodelphis domestica is described. Whole mounts of optic nerve, chiasm, and optic tract were sectioned horizontally and incubated with antibodies to myelin basic protein (MBP), proteolipid protein (PLP), myelin-associated glycoprotein (MAG), "Rip," and the neurite inhibitory protein (IN-1), followed by visualization with diaminobenzidine and a p ... [truncated at 450 characters in length]|
|Key phrase||Animals Biological Factors Immunohistochemistry Myelin Proteins Myelin Proteolipid Protein Myelin-Associated Glycoprotein Nerve Regeneration Opossums Optic Nerve Retinal Ganglion Cells Time Factors|
A modified silver staining protocol for visualization of proteins compatible with matrix-assisted laser desorption/ionization and electrospray ionization-mass spectrometry.
|Abstract||The growing availability of genomic sequence information, together with improvements in analytical methodology, have enabled high throughput, high sensitivity protein identification. Silver staining remains the most sensitive method for visualization of proteins separated by two-dimensional gel electrophoresis (2-D PAGE). Several silver staining protocols have been developed which offer improved compatibility with subsequent mass spectrometric an ... [truncated at 450 characters in length]|
|Author||Yan, JX; Wait, R; Berkelman, T; et al|
|Key phrase||Animals Humans Proteins Rats Silver Staining Spectrometry, Mass, Electrospray Ionization Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization|