Apoptosis and expression of DNA repair proteins in ischaemic brain injury in man.
|Abstract||We examined the timing of apoptosis and the expression of the DNA repair proteins poly(ADP-ribose) polymerase (PARP) and Ku80 in sections of frontal and temporal lobes from patients who had suffered severe brain ischaemia due to a cardiac arrent. In situ end-labelling (ISEL) was used to detect apoptotic cells, and immunohistochemistry to assess PARP and Ku80. ISEL of scattered neurons and glia was demonstrable predominantly during the first 24 h ... [truncated at 450 characters in length]|
|Author||Love, S; Barber, R; Wilcock, GK;|
|Key phrase||Adult Aged Antigens, Nuclear Apoptosis Brain Ischemia Child DNA Helicases DNA Repair DNA-Binding Proteins Female Frontal Lobe Humans Immunohistochemistry In Situ Hybridization Male Middle Aged Nerve Tissue Proteins Nuclear Proteins Poly(ADP-ribose) Polymerases Temporal Lobe|
Discovery and analysis of the first endogenous lentivirus.
|Abstract||The lentiviruses are associated with a wide range of chronic diseases in mammals. These include immunodeficiencies (such as HIV/AIDS in humans), malignancies, and lymphatic and neurological disorders in primates, felids, and a variety of wild and domesticated ungulates. Evolutionary analyses of the genomic sequences of modern-day lentiviruses have suggested a relatively recent date for their emergence, but the failure to identify any endogenous, ... [truncated at 450 characters in length]|
|Author||Katzourakis, A; Tristem, M; Pybus, OG; et al|
|Key phrase||Amino Acid Sequence Animals Base Sequence Computational Biology Endogenous Retroviruses Evolution, Molecular Genes, Viral Genome Genome Components Genomics Humans Lentivirus Likelihood Functions Models, Genetic Molecular Sequence Data Rabbits Sequence Analysis, DNA|
Isolation and characterisation of the crnA-niiA-niaD gene cluster for nitrate assimilation in Aspergillus nidulans.
|Abstract||Genomic clones containing the entire crnA-niiA-niaD gene cluster of Aspergillus nidulans have been isolated, and the structures of the niiA and niaD genes have been determined by nucleotide sequence analysis. This gene cluster is required for the assimilation of nitrate in A. nidulans, and the three genes encode a product required for nitrate uptake and the enzymes, nitrite reductase and nitrate reductase, respectively. The putative coding sequen ... [truncated at 450 characters in length]|
|Author||Johnstone, IL; McCabe, PC; Greaves, P; et al|
|Key phrase||Amino Acid Sequence Aspergillus nidulans Base Sequence Cloning, Molecular DNA, Fungal Molecular Sequence Data Multigene Family Mutation NADH, NADPH Oxidoreductases Nitrate Reductase Nitrate Reductases Nitrates Nitrite Reductases Nucleic Acid Hybridization Phenotype RNA, Messenger Restriction Mapping Transformation, Genetic|
Identification of a novel AU-rich-element-binding protein which is related to AUF1.
|Abstract||The AU-rich element (ARE) is an important instability determinant for a large number of early-response-gene mRNAs. AREs also mediate the stabilization of certain pro-inflammatory mRNAs, such as tumour necrosis factor (TNF)-alpha and cyclo-oxygenase-2 (COX-2), in response to inflammatory stimuli. To understand how AREs control mRNA stability, it is necessary to identify trans-acting factors. We have purified a new ARE-binding protein and identifie ... [truncated at 450 characters in length]|
|Author||Dean, JL; Sully, G; Wait, R; et al|
|Key phrase||3' Untranslated Regions Amino Acid Sequence Animals Blotting, Western Cell Line Chromatography Chromatography, High Pressure Liquid Cyclooxygenase 2 DNA-Binding Proteins Genes, Reporter HeLa Cells Heterogeneous-Nuclear Ribonucleoprotein D Heterogeneous-Nuclear Ribonucleoprotein Group A-B Humans Isoenzymes Macrophages Mass Spectrometry Membrane Proteins Mice Molecular Sequence Data Peptides Plasmids Precipitin Tests Prostaglandin-Endoperoxide Synthases Protein Binding Protein Isoforms RNA, Messenger RNA-Binding Proteins Recombinant Proteins Repressor Proteins Reverse Transcriptase Polymerase Chain Reaction Ribonucleases Sequence Homology, Amino Acid Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Subcellular Fractions Time Factors Tumor Necrosis Factor-alpha|
Repair of abasic sites in DNA.
|Abstract||Repair of both normal and reduced AP sites is activated by AP endonuclease, which recognizes and cleaves a phosphodiester bond 5' to the AP site. For a short period of time an incised AP site is occupied by poly(ADP-ribose) polymerase and then DNA polymerase beta adds one nucleotide into the repair gap and simultaneously removes the 5'-sugar phosphate. Finally, the DNA ligase III/XRCC1 complex accomplishes repair by sealing disrupted DNA ends. Ho ... [truncated at 450 characters in length]|
|Author||Dianov, GL; Sleeth, KM; Dianova, II; et al|
|Key phrase||Base Sequence DNA Repair Molecular Sequence Data|