Structure of the human RECQ1 helicase reveals a putative strand-separation pin.
|Abstract||RecQ-like helicases, which include 5 members in the human genome, are important in maintaining genome integrity. We present a crystal structure of a truncated form of the human RECQ1 protein with Mg-ADP. The truncated protein is active in DNA fork unwinding but lacks other activities of the full-length enzyme: disruption of Holliday junctions and DNA strand annealing. The structure of human RECQ1 resembles that of Escherichia coli RecQ, with some ... [truncated at 450 characters in length]|
|Author||Pike, Ashley C W; Shrestha, Binesh; Popuri, Venkateswarlu; et al|
|Subject||Adenosine Diphosphate Adenosine Triphosphate Amino Acid Motifs Amino Acid Sequence Conserved Sequence DNA Escherichia coli Humans Kinetics Molecular Sequence Data Mutant Proteins Protein Binding Protein Structure, Tertiary RecQ Helicases Sequence Alignment Zinc metabolism metabolism enzymology chemistry chemistry metabolism metabolism|
Superfamilies SDR and MDR: from early ancestry to present forms. Emergence of three lines, a Zn-metalloenzyme, and distinct variabilities.
|Abstract||Two large gene and protein superfamilies, SDR and MDR (short- and medium-chain dehydrogenases/reductases), were originally defined from analysis of alcohol and polyol dehydrogenases. The superfamilies contain minimally 82 and 25 genes, respectively, in humans, minimally 324 and 86 enzyme families when known lines in other organisms are also included, and over 47,000 and 15,000 variants in existing sequence data bank entries. SDR enzymes have one- ... [truncated at 450 characters in length]|
|Author||Jörnvall, Hans; Hedlund, Joel; Bergman, Tomas; et al|
|Subject||Acyl-CoA Dehydrogenase Butyryl-CoA Dehydrogenase Evolution, Molecular Humans Metalloproteins Phylogeny Protein Conformation Zinc chemistry classification genetics chemistry classification genetics chemistry classification genetics metabolism|