Dissecting the roles of XerC and XerD in Xer site-specific recombination
|Subject||Genetic recombination Tyrosine Research|
A study of tyrosine hydroxylase activity in nonadrenergic neurones in the rat brain
|Abstract||This abstract sent to supervisor 5/4/12 Tyrosine hydroxylase is the enzyme which controls the ratelimiting step in the synthesis of noradrenaline. In order to discover whether the activity of tyrosine hydroxylase might serve as an indicator of noradrenergic function in the brain, two preparations for the direct measurement of TH activity in rat brain regions by a tritium-release method were employed: synaptosomal suspensions prepared from pinched ... [truncated at 450 characters in length]|
|Subject||Tyrosine Tyrosine in the body Hydroxylation Noradrenergic neurons|
Role of aromatic localization in the gating process of a potassium channel.
|Abstract||Position of the transmembrane aromatic residues of the KirBac1.1 potassium channel shifts from an even distribution in the closed state toward the membrane/solute interface in the open state model. This is the first example of an integral membrane protein making use of the observed preference for transmembrane aromatic residues to reside at the interfaces. The process of aromatic localization is proposed as a means of directing and stabilizing st ... [truncated at 450 characters in length]|
|Author||Domene, Carmen; Vemparala, Satyavani; Klein, Michael L; et al|
|Subject||Biophysics Cell Membrane Computer Simulation G Protein-Coupled Inwardly-Rectifying Potassium Channels Ion Channel Gating Lipid Bilayers Lipids Models, Molecular Molecular Conformation Phenylalanine Potassium Channels Time Factors Tyrosine methods metabolism chemistry chemistry chemistry chemistry chemistry chemistry|
Three-dimensional structure and enzymatic function of proapoptotic human p53-inducible quinone oxidoreductase PIG3.
|Abstract||Tumor suppressor p53 regulates the expression of p53-induced genes (PIG) that trigger apoptosis. PIG3 or TP53I3 is the only known member of the medium chain dehydrogenase/reductase superfamily induced by p53 and is used as a proapoptotic marker. Although the participation of PIG3 in the apoptotic pathway is proven, the protein and its mechanism of action were never characterized. We analyzed human PIG3 enzymatic function and found NADPH-dependent ... [truncated at 450 characters in length]|
|Author||Porté, Sergio; Valencia, Eva; Yakovtseva, Evgenia A; et al|
|Subject||Amino Acid Sequence Apoptosis Binding Sites Catalytic Domain Crystallography, X-Ray Humans Intracellular Signaling Peptides and Proteins Kinetics Models, Molecular Molecular Sequence Data Mutagenesis, Site-Directed NADP Phylogeny Protein Structure, Quaternary Proto-Oncogene Proteins Reactive Oxygen Species Recombinant Proteins Sequence Homology, Amino Acid Tumor Suppressor Protein p53 Tyrosine physiology genetics chemistry genetics metabolism metabolism chemistry genetics metabolism metabolism chemistry genetics metabolism metabolism chemistry|
Selective inhibitors of the JMJD2 histone demethylases: combined nondenaturing mass spectrometric screening and crystallographic approaches.
|Abstract||Ferrous ion and 2-oxoglutarate (2OG) oxygenases catalyze the demethylation of N(epsilon)-methylated lysine residues in histones. Here we report studies on the inhibition of the JMJD2 subfamily of histone demethylases, employing binding analyses by nondenaturing mass spectrometry (MS), dynamic combinatorial chemistry coupled to MS, turnover assays, and crystallography. The results of initial binding and inhibition assays directed the production an ... [truncated at 450 characters in length]|
|Author||Rose, Nathan R; Woon, Esther C Y; Kingham, Guy L; et al|
|Subject||Binding Sites Binding, Competitive Combinatorial Chemistry Techniques Crystallography, X-Ray Humans Jumonji Domain-Containing Histone Demethylases Models, Molecular Oxalic Acids Spectrometry, Mass, Electrospray Ionization Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Structure-Activity Relationship Tyrosine antagonists and inhibitors chemistry chemical synthesis chemistry analogs and derivatives chemical synthesis chemistry|