Dynamic protein methylation in chromatin biology.
|Abstract||Post-translational modification of chromatin is emerging as an increasingly important regulator of chromosomal processes. In particular, histone lysine and arginine methylation play important roles in regulating transcription, maintaining genomic integrity, and contributing to epigenetic memory. Recently, the use of new approaches to analyse histone methylation, the generation of genetic model systems, and the ability to interrogate genome wide h ... [truncated at 450 characters in length]|
|Author||Ng, S S; Yue, W W; Oppermann, U; et al|
|Subject||Animals Arginine Chromatin DNA Damage Embryonic Stem Cells Epigenesis, Genetic Gene Expression Regulation Germ Cells Histone-Lysine N-Methyltransferase Histones Lysine Methylation Models, Molecular Molecular Structure Neoplasms Oxidoreductases, N-Demethylating Protein Conformation Protein Methyltransferases Protein Processing, Post-Translational Tumor Suppressor Protein p53 metabolism metabolism physiology physiology genetics metabolism metabolism genetics metabolism genetics metabolism chemistry genetics metabolism genetics metabolism|
Three-dimensional structure and enzymatic function of proapoptotic human p53-inducible quinone oxidoreductase PIG3.
|Abstract||Tumor suppressor p53 regulates the expression of p53-induced genes (PIG) that trigger apoptosis. PIG3 or TP53I3 is the only known member of the medium chain dehydrogenase/reductase superfamily induced by p53 and is used as a proapoptotic marker. Although the participation of PIG3 in the apoptotic pathway is proven, the protein and its mechanism of action were never characterized. We analyzed human PIG3 enzymatic function and found NADPH-dependent ... [truncated at 450 characters in length]|
|Author||Porté, Sergio; Valencia, Eva; Yakovtseva, Evgenia A; et al|
|Subject||Amino Acid Sequence Apoptosis Binding Sites Catalytic Domain Crystallography, X-Ray Humans Intracellular Signaling Peptides and Proteins Kinetics Models, Molecular Molecular Sequence Data Mutagenesis, Site-Directed NADP Phylogeny Protein Structure, Quaternary Proto-Oncogene Proteins Reactive Oxygen Species Recombinant Proteins Sequence Homology, Amino Acid Tumor Suppressor Protein p53 Tyrosine physiology genetics chemistry genetics metabolism metabolism chemistry genetics metabolism metabolism chemistry genetics metabolism metabolism chemistry|
Conformational stability and activity of p73 require a second helix in the tetramerization domain.
|Abstract||p73 and p63, the two ancestral members of the p53 family, are involved in neurogenesis, epithelial stem cell maintenance and quality control of female germ cells. The highly conserved oligomerization domain (OD) of tumor suppressor p53 is essential for its biological functions, and its structure was believed to be the prototype for all three proteins. However, we report that the ODs of p73 and p63 differ from the OD of p53 by containing an additi ... [truncated at 450 characters in length]|
|Author||Coutandin, D; Löhr, F; Niesen, F H; et al|
|Subject||Amino Acid Sequence Animals Conserved Sequence DNA-Binding Proteins Humans Mice Models, Molecular Molecular Sequence Data Mutation Nuclear Magnetic Resonance, Biomolecular Nuclear Proteins Phosphoproteins Protein Multimerization Protein Structure, Quaternary Protein Structure, Secondary Sequence Alignment Thermodynamics Trans-Activators Tumor Suppressor Protein p53 Tumor Suppressor Proteins chemistry genetics metabolism chemistry genetics metabolism chemistry metabolism chemistry metabolism chemistry metabolism chemistry genetics metabolism|