Structural determinants of G-protein alpha subunit selectivity by regulator of G-protein signaling 2 (RGS2).
|Abstract||"Regulator of G-protein signaling" (RGS) proteins facilitate the termination of G protein-coupled receptor (GPCR) signaling via their ability to increase the intrinsic GTP hydrolysis rate of Galpha subunits (known as GTPase-accelerating protein or "GAP" activity). RGS2 is unique in its in vitro potency and selectivity as a GAP for Galpha(q) subunits. As many vasoconstrictive hormones signal via G(q) heterotrimer-coupled receptors, it is perhaps n ... [truncated at 450 characters in length]|
|Author||Kimple, Adam J; Soundararajan, Meera; Hutsell, Stephanie Q; et al|
|Subject||Binding Sites Cell Line Evolution, Molecular Fluorescence Resonance Energy Transfer GTP-Binding Protein alpha Subunits Guanosine Triphosphate Humans Hydrolysis Luminescent Proteins Models, Molecular Point Mutation Protein Binding Protein Interaction Domains and Motifs Protein Structure, Tertiary RGS Proteins Recombinant Fusion Proteins Surface Plasmon Resonance Transfection genetics genetics metabolism metabolism genetics metabolism chemistry genetics metabolism chemistry genetics metabolism|