Expression and purification of recombinant human inward rectifier K+ (KCNJ) channels in Saccharomyces cerevisiae.
|Abstract||The inward rectifier family of potassium (KCNJ) channels regulate vital cellular processes including cell volume, electrical excitability, and insulin secretion. Dysfunction of different isoforms have been linked to numerous diseases including Bartter's, Andersen-Tawil, Smith-Magenis Syndromes, Type II diabetes mellitus, and epilepsy, making them important targets for therapeutic intervention. Using a family-based approach, we succeeded in expres ... [truncated at 450 characters in length]|
|Author||D'Avanzo, Nazzareno; Cheng, Wayland W L; Xia, Xiaobing; et al|
|Subject||Biochemistry Cloning, Molecular Humans Potassium Channels, Inwardly Rectifying Protein Transport Recombinant Proteins Saccharomyces cerevisiae Subcellular Fractions methods isolation and purification metabolism isolation and purification metabolism metabolism metabolism|