Experiments designed to study the movement of genes between the plasmids and the chromosome of Staphylococcus aureus are described.
Two derivatives of the plasmid pI9789::Tn552, that are temperature-sensitive for replication, pSl and pAD, are used as a model system for this investigation. The plasmids carry a gene conferring resistance to cadmium salts and so the number of cadmium-resistant colonies appearing on selective plates ... [truncated at 450 characters in length]
|Subject||Plasmids Genetics Chromosomes Staphylococcus aureus|
|Abstract||Sirtuins are a family of protein lysine deacetylases, which regulate gene silencing, metabolism, life span, and chromatin structure. Sirtuins utilize NAD(+) to deacetylate proteins, yielding O-acetyl-ADP-ribose (OAADPr) as a reaction product. The macrodomain is a ubiquitous protein module known to bind ADP-ribose derivatives, which diverged through evolution to support many different protein functions and pathways. The observation that some sirtu ... [truncated at 450 characters in length]|
|Author||Chen, Dawei; Vollmar, Melanie; Rossi, Marianna N; et al|
|Subject||Escherichia coli Escherichia coli Proteins Evolution, Molecular Fungal Proteins Hela Cells Humans Neurospora crassa Protein Structure, Tertiary Sirtuins Staphylococcus aureus enzymology genetics chemistry genetics metabolism chemistry genetics metabolism enzymology genetics chemistry genetics metabolism enzymology genetics|