Ligand supplementation as a method to increase soluble heterologous protein production.
|Abstract||Ligand interactions are central to enzyme or receptor function, constituting a cornerstone in biochemistry and pharmacology. Here we discuss a ligand application that can be exploited to significantly increase the proportion of recombinant protein expressed in soluble form, by including ligands during the culture. Provided that a sufficiently soluble, cell-permeable and avid ligand is available, one can use it to stabilize nascently synthesized p ... [truncated at 450 characters in length]|
|Author||Hozjan, Viktorija; Guo, Kunde; Wu, Xiaoqiu; et al|
|Subject||11-beta-Hydroxysteroid Dehydrogenase Type 1 Carbenoxolone Cell Membrane Permeability Cloning, Molecular Escherichia coli Glycyrrhetinic Acid Humans Ligands Oxidoreductases Protein Binding Recombinant Proteins Solubility biosynthesis genetics pharmacology methods metabolism pharmacology biosynthesis genetics biosynthesis|