Protein-protein interaction site mapping using NMR-detected mutational scanning.
|Abstract||We demonstrate a novel NMR method for the mapping of protein-protein interaction sites. In our approach protein-protein binding sites are mapped by competition binding experiments using indirect NMR reporter technology and Ala positional scanning. The methodology provides high sensitivity, ease of implementation and high-throughput capabilities. The feasibility of the technique is demonstrated with an application to the beta-Catenin/Tcf4 complex.|
|Author||Baminger, Bettina; Ludwiczek, Martin L; Kontaxis, Georg; et al|
|Subject||Binding, Competitive Humans Ligands Models, Molecular Mutation Nuclear Magnetic Resonance, Biomolecular Protein Interaction Mapping Reproducibility of Results TCF Transcription Factors Transcription Factor 7-Like 2 Protein beta Catenin genetics methods methods metabolism metabolism|