High-throughput production of human proteins for crystallization: the SGC experience.
|Abstract||Producing purified human proteins with high yield and purity remains a considerable challenge. We describe the methods utilized in the Structural Genomics Consortium (SGC) in Oxford, resulting in successful purification of 48% of human proteins attempted; of those, the structures of approximately 40% were solved by X-ray crystallography. The main driver has been the parallel processing of multiple (typically 9-20) truncated constructs of each tar ... [truncated at 450 characters in length]|
|Author||Savitsky, Pavel; Bray, James; Cooper, Christopher D O; et al|
|Subject||Amino Acid Sequence Animals Cell Line Cloning, Molecular Crystallography, X-Ray Genetic Vectors Genomics Humans Molecular Sequence Data Proteins Proteomics Recombinant Proteins genetics methods chemistry genetics metabolism methods chemistry isolation and purification metabolism|