|Author||Shiu, Stephen Yuen Wing.;|
|Subject||Louping ill Molecular aspects Tick-borne encephalitis viruses|
The recognition of MHC-peptide ligands by the T cell receptor (TCR) is central to the induction of the adaptive immune response. This thesis describes the development of a bioassay for TCR recognition which was then used to undertake a molecular analysis of the TCR/MHC-peptide interaction.
1. A TCR-CD3ϛ chimeric receptor was stably expressed in the cell line RBL-2H3 to give the transfectant RBL-008. RBL-008 was shown to exhibit MHC-res ... [truncated at 450 characters in length]
|Author||Vessey, S. J. R.;|
|Subject||T cells Receptors Molecular aspects Analysis|
Genomic clones were isolated and characterized using the human monoamine oxidase A (MAOA) cDNA to screen a phage library, constructed from a human 4X cell line (48, XXXX). The genomic contig derived from overlapping phage clones showed that the size of the MAOA gene is over 80 kb. Exon-containing fragments from these phage clones were subcloned and sequenced. The data from this showed that the MAOA gene consists of 15 exons.
A YAC (yea ... [truncated at 450 characters in length]
|Subject||X chromosome Molecular aspects Abnormalities Genetic disorders Monoamine oxidase|
|Author||Bradshaw, Jeremy Peter.;|
|Subject||Macromolecules Structure Connective tissues Diseases Molecular aspects Collagen|
X-chromosome inactivation achieves dosage compensation of X-linked genes between male (XY) and female (XX) mammals. This process involves the down-regulation of most, but not all genes on one of the two X-chromosomes in the nucleus of each female somatic cell. The mechanism of X-inactivation has yet to be elucidated in full, but is known to involve the noncoding transcript of theXist gene, DNA methylation, histone hypo-acetylation and the con ... [truncated at 450 characters in length]
|Author||Simpson T. Ian.;|
|Subject||Gene silencing X chromosome Molecular aspects|