High-performance liquid chromatography separation and intact mass analysis of detergent-solubilized integral membrane proteins.
|Abstract||We have developed a method for intact mass analysis of detergent-solubilized and purified integral membrane proteins using liquid chromatography-mass spectrometry (LC-MS) with methanol as the organic mobile phase. Membrane proteins and detergents are separated chromatographically during the isocratic stage of the gradient profile from a 150-mm C3 reversed-phase column. The mass accuracy is comparable to standard methods employed for soluble prote ... [truncated at 450 characters in length]|
|Author||Berridge, Georgina; Chalk, Rod; D'Avanzo, Nazzareno; et al|
|Subject||Chromatography Chromatography, High Pressure Liquid Chromatography, Liquid Detergents Mass Spectrometry Membrane Proteins Methanol Spectrometry, Mass, Electrospray Ionization instrumentation methods methods methods chemistry methods chemistry chemistry methods|