Structure and regulation of the human Nek2 centrosomal kinase.
|Abstract||The dimeric Ser/Thr kinase Nek2 regulates centrosome cohesion and separation through phosphorylation of structural components of the centrosome, and aberrant regulation of Nek2 activity can lead to aneuploid defects characteristic of cancer cells. Mutational analysis of autophosphorylation sites within the kinase domain identified by mass spectrometry shows a complex pattern of positive and negative regulatory effects on kinase activity that are ... [truncated at 450 characters in length]|
|Author||Rellos, Peter; Ivins, Frank J; Baxter, Joanne E; et al|
|Subject||Allosteric Regulation Binding Sites Centrosome Crystallography, X-Ray DNA Mutational Analysis Dimerization Humans Mass Spectrometry Phosphorylation Protein-Serine-Threonine Kinases enzymology antagonists and inhibitors chemistry|
High-performance liquid chromatography separation and intact mass analysis of detergent-solubilized integral membrane proteins.
|Abstract||We have developed a method for intact mass analysis of detergent-solubilized and purified integral membrane proteins using liquid chromatography-mass spectrometry (LC-MS) with methanol as the organic mobile phase. Membrane proteins and detergents are separated chromatographically during the isocratic stage of the gradient profile from a 150-mm C3 reversed-phase column. The mass accuracy is comparable to standard methods employed for soluble prote ... [truncated at 450 characters in length]|
|Author||Berridge, Georgina; Chalk, Rod; D'Avanzo, Nazzareno; et al|
|Subject||Chromatography Chromatography, High Pressure Liquid Chromatography, Liquid Detergents Mass Spectrometry Membrane Proteins Methanol Spectrometry, Mass, Electrospray Ionization instrumentation methods methods methods chemistry methods chemistry chemistry methods|