Aldehyde dehydrogenase 7A1 (ALDH7A1) is a novel enzyme involved in cellular defense against hyperosmotic stress.
|Abstract||Mammalian ALDH7A1 is homologous to plant ALDH7B1, an enzyme that protects against various forms of stress, such as salinity, dehydration, and osmotic stress. It is known that mutations in the human ALDH7A1 gene cause pyridoxine-dependent and folic acid-responsive seizures. Herein, we show for the first time that human ALDH7A1 protects against hyperosmotic stress by generating osmolytes and metabolizing toxic aldehydes. Human ALDH7A1 expression in ... [truncated at 450 characters in length]|
|Author||Brocker, Chad; Lassen, Natalie; Estey, Tia; et al|
|Subject||Aldehyde Dehydrogenase Aldehydes Animals CHO Cells Cricetinae Cricetulus Crystallography, X-Ray Female Gene Expression Regulation, Enzymologic Humans Male Mice Osmosis Proteins Tissue Distribution metabolism physiology chemistry methods metabolism physiology|
Quinone oxidoreductase-2-mediated prodrug cancer therapy.
|Abstract||DNA-damaging agents are widely used in cancer treatment despite their lack of tumor specificity. Human NQO2 (quinone oxidoreductase-2) is an atypical oxidoreductase because no endogenous electron donor has been identified to date. The enzyme converts CB1954 [5-(aziridin-1-yl)-2,4-dinitrobenzamide], in the presence of the synthetic nicotinamide cofactor analog EP0152R, to a cytotoxic bifunctional alkylating agent. NQO2 activity in hepatocellular t ... [truncated at 450 characters in length]|
|Author||Middleton, Mark R; Knox, Richard; Cattell, Emma; et al|
|Subject||Adult Aged Antineoplastic Agents Aziridines Cell Death Cross-Linking Reagents Crystallography, X-Ray DNA, Neoplasm Enzyme Activation Female Humans Male Middle Aged Models, Molecular Neoplasms Prodrugs Quinone Reductases adverse effects chemistry pharmacokinetics therapeutic use adverse effects chemistry pharmacokinetics therapeutic use drug effects pharmacology metabolism drug effects drug therapy chemistry pharmacology therapeutic use chemistry metabolism|