Development of homogeneous luminescence assays for histone demethylase catalysis and binding.
|Abstract||Covalent modifications to histones play important roles in chromatin dynamics and the regulation of gene expression. The JumonjiC (JmjC)-containing histone demethylases (HDMs) catalyze the demethylation of methylated lysine residues on histone tails. Here we report the development of homogeneous luminescence-based assay methods for measuring the catalytic activity and the binding affinities of peptides to HDMs. The assays use amplified luminescen ... [truncated at 450 characters in length]|
|Author||Kawamura, Akane; Tumber, Anthony; Rose, Nathan R; et al|
|Subject||Biocatalysis Enzyme Inhibitors Humans Jumonji Domain-Containing Histone Demethylases Luminescent Measurements Peptides Protein Binding Protein Isoforms Protein Structure, Tertiary chemistry pharmacology metabolism methods chemistry metabolism|