Structures of the human eIF4E homologous protein, h4EHP, in its m7GTP-bound and unliganded forms.
|Abstract||All eukaryotic cellular mRNAs contain a 5' m(7)GpppN cap. In addition to conferring stability to the mRNA, the cap is required for pre-mRNA splicing, nuclear export and translation by providing an anchor point for protein binding. In translation, the interaction between the cap and the eukaryotic initiation factor 4E (eIF4E) is important in the recruitment of the mRNAs to the ribosome. Human 4EHP (h4EHP) is a homologue of eIF4E. Like eIF4E it is ... [truncated at 450 characters in length]|
|Author||Rosettani, Pamela; Knapp, Stefan; Vismara, Maria-Grazia; et al|
|Subject||Amino Acid Sequence Binding Sites Calorimetry Conserved Sequence Crystallography, X-Ray Eukaryotic Initiation Factor-4E Evolution, Molecular Guanosine Triphosphate Humans Models, Molecular Molecular Sequence Data Mutation Peptides Protein Binding RNA Cap-Binding Proteins RNA Caps chemistry genetics metabolism chemistry metabolism chemistry chemistry genetics metabolism chemistry|
Bisphosphonates: an update on mechanisms of action and how these relate to clinical efficacy.
|Abstract||The bisphosphonates (BPs) are well established as the treatments of choice for disorders of excessive bone resorption, including Paget's disease of bone, myeloma and bone metastases, and osteoporosis. There is considerable new knowledge about how BPs work. Their classical pharmacological effects appear to result from two key properties: their affinity for bone mineral and their inhibitory effects on osteoclasts. Mineral binding affinities differ ... [truncated at 450 characters in length]|
|Author||Russell, R Graham G; Xia, Zhidao; Dunford, James E; et al|
|Subject||Animals Bone Neoplasms Bone Resorption Bone and Bones Diphosphonates Guanosine Triphosphate Humans Models, Biological Models, Chemical Multiple Myeloma Neoplasm Metastasis Nitrogen Osteoclasts Osteocytes Osteoporosis Protein Processing, Post-Translational T-Lymphocytes Treatment Outcome secondary metabolism chemistry pharmacology therapeutic use chemistry metabolism chemistry metabolism metabolism therapy metabolism|
Structural determinants of G-protein alpha subunit selectivity by regulator of G-protein signaling 2 (RGS2).
|Abstract||"Regulator of G-protein signaling" (RGS) proteins facilitate the termination of G protein-coupled receptor (GPCR) signaling via their ability to increase the intrinsic GTP hydrolysis rate of Galpha subunits (known as GTPase-accelerating protein or "GAP" activity). RGS2 is unique in its in vitro potency and selectivity as a GAP for Galpha(q) subunits. As many vasoconstrictive hormones signal via G(q) heterotrimer-coupled receptors, it is perhaps n ... [truncated at 450 characters in length]|
|Author||Kimple, Adam J; Soundararajan, Meera; Hutsell, Stephanie Q; et al|
|Subject||Binding Sites Cell Line Evolution, Molecular Fluorescence Resonance Energy Transfer GTP-Binding Protein alpha Subunits Guanosine Triphosphate Humans Hydrolysis Luminescent Proteins Models, Molecular Point Mutation Protein Binding Protein Interaction Domains and Motifs Protein Structure, Tertiary RGS Proteins Recombinant Fusion Proteins Surface Plasmon Resonance Transfection genetics genetics metabolism metabolism genetics metabolism chemistry genetics metabolism chemistry genetics metabolism|
Structures of the human GTPase MMAA and vitamin B12-dependent methylmalonyl-CoA mutase and insight into their complex formation.
|Abstract||Vitamin B(12) (cobalamin, Cbl) is essential to the function of two human enzymes, methionine synthase (MS) and methylmalonyl-CoA mutase (MUT). The conversion of dietary Cbl to its cofactor forms, methyl-Cbl (MeCbl) for MS and adenosyl-Cbl (AdoCbl) for MUT, located in the cytosol and mitochondria, respectively, requires a complex pathway of intracellular processing and trafficking. One of the processing proteins, MMAA (methylmalonic aciduria type ... [truncated at 450 characters in length]|
|Author||Froese, D Sean; Kochan, Grazyna; Muniz, João R C; et al|
|Subject||Child Child, Preschool Cobamides Crystallography, X-Ray Cytosol Guanosine Diphosphate Guanosine Triphosphate Humans Membrane Transport Proteins Metabolism, Inborn Errors Methylmalonyl-CoA Mutase Mitochondria Mitochondrial Proteins Multiprotein Complexes Mutation, Missense Protein Structure, Quaternary chemistry genetics metabolism chemistry metabolism chemistry genetics metabolism chemistry genetics metabolism chemistry genetics metabolism chemistry genetics metabolism chemistry genetics metabolism chemistry genetics metabolism chemistry genetics metabolism|