Baculovirus expression vector system: an emerging host for high-throughput eukaryotic protein expression.
|Abstract||The increasing demand for production and characterization of diverse groups of recombinant proteins necessitates the analysis of several constructs and fusion tags in a variety of expression systems. The challenge is to screen multiple clones quickly for the desired properties. When using a eukaryotic system, such as baculovirus-mediated expression in insect cells, the total time required and the volume of culture needed to obtain reasonable resu ... [truncated at 450 characters in length]|
|Author||Shrestha, Binesh; Smee, Carol; Gileadi, Opher;|
|Subject||Animals Baculoviridae Base Sequence Cell Line DNA Primers DNA, Viral Genetic Vectors Polymerase Chain Reaction Recombinant Proteins Spodoptera genetics genetics genetics|
High-throughput production of human proteins for crystallization: the SGC experience.
|Abstract||Producing purified human proteins with high yield and purity remains a considerable challenge. We describe the methods utilized in the Structural Genomics Consortium (SGC) in Oxford, resulting in successful purification of 48% of human proteins attempted; of those, the structures of approximately 40% were solved by X-ray crystallography. The main driver has been the parallel processing of multiple (typically 9-20) truncated constructs of each tar ... [truncated at 450 characters in length]|
|Author||Savitsky, Pavel; Bray, James; Cooper, Christopher D O; et al|
|Subject||Amino Acid Sequence Animals Cell Line Cloning, Molecular Crystallography, X-Ray Genetic Vectors Genomics Humans Molecular Sequence Data Proteins Proteomics Recombinant Proteins genetics methods chemistry genetics metabolism methods chemistry isolation and purification metabolism|