Baculovirus expression vector system: an emerging host for high-throughput eukaryotic protein expression.
|Abstract||The increasing demand for production and characterization of diverse groups of recombinant proteins necessitates the analysis of several constructs and fusion tags in a variety of expression systems. The challenge is to screen multiple clones quickly for the desired properties. When using a eukaryotic system, such as baculovirus-mediated expression in insect cells, the total time required and the volume of culture needed to obtain reasonable resu ... [truncated at 450 characters in length]|
|Author||Shrestha, Binesh; Smee, Carol; Gileadi, Opher;|
|Subject||Animals Baculoviridae Base Sequence Cell Line DNA Primers DNA, Viral Genetic Vectors Polymerase Chain Reaction Recombinant Proteins Spodoptera genetics genetics genetics|
Analysis of the substrate-binding site of human carbonyl reductases CBR1 and CBR3 by site-directed mutagenesis.
|Abstract||Human carbonyl reductase is a member of the short-chain dehydrogenase/reductase (SDR) protein superfamily and is known to play an important role in the detoxification of xenobiotics bearing a carbonyl group. The two monomeric NADPH-dependent human isoforms of cytosolic carbonyl reductase CBR1 and CBR3 show a sequence similarity of 85% on the amino acid level, which is definitely high if compared to the low similarities usually observed among othe ... [truncated at 450 characters in length]|
|Author||El-Hawari, Yasser; Favia, Angelo D; Pilka, Ewa S; et al|
|Subject||Alcohol Oxidoreductases Amino Acid Sequence Base Sequence Binding Sites Biocatalysis DNA Primers DNA, Complementary Humans Kinetics Models, Molecular Molecular Sequence Data Mutagenesis, Site-Directed Sequence Homology, Amino Acid Substrate Specificity chemistry genetics isolation and purification metabolism|
A novel ACVR1 mutation in the glycine/serine-rich domain found in the most benign case of a fibrodysplasia ossificans progressiva variant reported to date.
|Abstract||Fibrodysplasia Ossificans Progressiva (FOP) is a rare, autosomal dominant condition, classically characterised by heterotopic ossification beginning in childhood and congenital great toe malformations; occurring in response to a c.617 G > A ACVR1 mutation in the functionally important glycine/serine-rich domain of exon 6. Here we describe a novel c.587 T > C mutation in the glycine/serine-rich domain of ACVR1, associated with delayed onset of het ... [truncated at 450 characters in length]|
|Author||Gregson, Celia L; Hollingworth, Peter; Williams, Martin; et al|
|Subject||Activin Receptors, Type I Adult Base Sequence DNA Mutational Analysis Female Heterozygote Humans Middle Aged Molecular Sequence Data Mutation Myositis Ossificans Ossification, Heterotopic Protein Structure, Tertiary Young Adult chemistry genetics genetics complications genetics complications genetics|
Crystal structure of the 2-oxoglutarate- and Fe(II)-dependent lysyl hydroxylase JMJD6.
|Abstract||Lysyl and prolyl hydroxylations are well-known post-translational modifications to animal and plant proteins with extracellular roles. More recent work has indicated that the hydroxylation of intracellular animal proteins may be common. JMJD6 catalyses the iron- and 2-oxoglutarate-dependent hydroxylation of lysyl residues in arginine-serine-rich domains of RNA splicing-related proteins. We report crystallographic studies on the catalytic domain o ... [truncated at 450 characters in length]|
|Author||Mantri, Monica; Krojer, Tobias; Bagg, Eleanor A; et al|
|Subject||Amino Acid Sequence Amino Acid Substitution Base Sequence Catalytic Domain Crystallography, X-Ray DNA Primers Humans Iron Jumonji Domain-Containing Histone Demethylases Ketoglutaric Acids Models, Molecular Molecular Sequence Data Mutagenesis, Site-Directed Mutant Proteins Nickel Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase Protein Folding Recombinant Proteins Sequence Homology, Amino Acid Static Electricity genetics metabolism chemistry genetics metabolism metabolism chemistry genetics metabolism metabolism chemistry genetics metabolism chemistry genetics metabolism|