Carbonyl reductases: the complex relationships of mammalian carbonyl- and quinone-reducing enzymes and their role in physiology.
|Abstract||Carbonyl groups are frequently found in endogenous or xenobiotic compounds. Reactive carbonyls, formed during lipid peroxidation or food processing, and xenobiotic quinones are able to covalently modify DNA or amino acids. They can also promote oxidative stress, the products of which are thought to be an important initiating factor in degenerative diseases or cancer. Carbonyl groups are reduced by an array of distinct NADPH-dependent enzymes, bel ... [truncated at 450 characters in length]|
|Subject||Alcohol Oxidoreductases Animals Humans Mammals NADH, NADPH Oxidoreductases Oxidative Stress Quinones Structure-Activity Relationship Xenobiotics metabolism physiology metabolism metabolism|
Analysis of the substrate-binding site of human carbonyl reductases CBR1 and CBR3 by site-directed mutagenesis.
|Abstract||Human carbonyl reductase is a member of the short-chain dehydrogenase/reductase (SDR) protein superfamily and is known to play an important role in the detoxification of xenobiotics bearing a carbonyl group. The two monomeric NADPH-dependent human isoforms of cytosolic carbonyl reductase CBR1 and CBR3 show a sequence similarity of 85% on the amino acid level, which is definitely high if compared to the low similarities usually observed among othe ... [truncated at 450 characters in length]|
|Author||El-Hawari, Yasser; Favia, Angelo D; Pilka, Ewa S; et al|
|Subject||Alcohol Oxidoreductases Amino Acid Sequence Base Sequence Binding Sites Biocatalysis DNA Primers DNA, Complementary Humans Kinetics Models, Molecular Molecular Sequence Data Mutagenesis, Site-Directed Sequence Homology, Amino Acid Substrate Specificity chemistry genetics isolation and purification metabolism|
Structural basis for substrate specificity in human monomeric carbonyl reductases.
|Abstract||Carbonyl reduction constitutes a phase I reaction for many xenobiotics and is carried out in mammals mainly by members of two protein families, namely aldo-keto reductases and short-chain dehydrogenases/reductases. In addition to their capacity to reduce xenobiotics, several of the enzymes act on endogenous compounds such as steroids or eicosanoids. One of the major carbonyl reducing enzymes found in humans is carbonyl reductase 1 (CBR1) with a v ... [truncated at 450 characters in length]|
|Author||Pilka, Ewa S; Niesen, Frank H; Lee, Wen Hwa; et al|
|Subject||Alcohol Oxidoreductases Antineoplastic Agents Cloning, Molecular Crystallography, X-Ray Ethanolamines Humans Isoquinolines Kinetics Mutagenesis Mutagenesis, Site-Directed Structure-Activity Relationship Substrate Specificity Temperature Xenobiotics chemistry pharmacology methods chemistry chemistry chemistry|